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Application of microarrays in high-throughput enzymatic profiling

✍ Scribed by Mahesh Uttamchandani; Xuan Huang; Grace Y. J. Chen; Lay-Pheng Tan; Shao Q. Yao

Book ID
111640436
Publisher
Humana Press Inc
Year
2004
Tongue
English
Weight
480 KB
Volume
28
Category
Article
ISSN
1073-6085

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MicroRNAs (miRNAs) are small regulatory RNAs that serve fundamental biological roles across eukaryotic species. We describe a new method for high-throughput miRNA detection. The technique is termed the RNA-primed, array-based Klenow enzyme (RAKE) assay, because it involves on-slide application of th