Application of liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry for screening and quantitative analysis of C21 steroids in the roots and rhizomes of Cynanchum paniculatum

✍ Scribed by Jing Dou; Ping Li; Yue Song; Lian-Wen Qi; Zhi-Ming Bi

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 617 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600434

✦ Synopsis

Abstract

A liquid chromatography‐mass spectrometry method is presented for the quantification of C~21~ steroids in the roots and rhizomes of Cynanchum paniculatum. Eight C~21~ steroids, including five steroidal aglycones and three steroidal glycosides, were simultaneously analyzed by liquid chromatography coupled with electrospray ionization time‐of‐flight mass spectrometry. The extracted ion current chromatograms were extracted from the total ion current chromatogram using characteristic ions produced by target compounds for peak determination. Chromatographic separation was achieved on a C~18~ reversed‐phase column within 60 min, using an acetonitrile/water gradient. For comparision, six C. paniculatum samples from different locations were investigated by the established method, and the results indicated that the different geographical origin significantly influenced the C~21~ steroid composition. The method was observed to have the necessary sensitivity, selectivity, precision, and accuracy, and to be suitable for quality control of herbal medicines and their preparations.