Application of laser scan microscopy in vivo for wound healing characterization

✍ Scribed by V. Czaika; A. Alborova; W. Sterry; J. Lademann; S. Koch

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 426 KB
• Volume: 7
• Category: Article
• ISSN: 1612-2011
• DOI: 10.1002/lapl.201010038

✦ Synopsis

Considering the advancing age of the population, wound healing disturbances are becoming increasingly important in clinical routine. The development of wound healing creams and lotions as well as therapy control require an objective evaluation of the wound healing process, which represents the destruction of the barrier. Therefore, transepidermal water loss measurements are often carried out. These measurements have the disadvantage that they are disturbed by the interstitial fluid, which is located on the surface of chronic wounds and also by water components of the creams and lotions. Additionally, the TEWL measurements are very sensitive to temperature changes and to the anxiety of the volunteers.

In the present study, in vivo laser scanning microscopy was used to analyze the reepithelialization and barrier recovery of standardized wounds produced by the suction blister technique. It was demonstrated that this non-invasive, on-line spectroscopic method allows the evaluation of the wound healing process, without any disturbances. It was found that the wound healing starts not only from the edges of the wound, but also out of the hair follicles. The in vivo laser scanning microscopy is well suited to evaluate the efficacy of wound healing creams and for therapy control. 50 μm SC SG Boundary between the stratum corneum (SC) and the stratum granulosum (SG)