Application of isothermal titration calorimetry for detecting lipid membrane solubilization
✍ Scribed by H. Heerklotz; G. Lantzsch; H. Binder; G. Klose; A. Blume
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 245 KB
- Volume
- 235
- Category
- Article
- ISSN
- 0009-2614
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✦ Synopsis
Isothermal titration calorimetry is presented to be an excellent method of detecting lipid membrane solubilization upon the addition of detergents. As an example, the titration of the nan-ionic detergent C t2EOt, to the lipid POPC is studied. The main contribution to the measured titration heat is the detergent's enthalpy difference upon transfer between micelles and membranes. The detergent added as micelles is transferred into membranes up to the saturating detergent per lipid mol/mol ratio, Rsa t = 1.45 + 0.07. Beyond Rsa t the membranes are solubilized to micelles. Subsequently, the titration heat changes its sign from endothermic to exothermic. After complete membrane dissolution at Rso ~ = 5.0 + 0.4 the titration heat vanishes nearly completely.