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Application of ion-pair high-performance liquid chromatography to detection of the atypical coproporphyrin isomers II and IV in human faeces

โœ Scribed by K. Jacob; E. Egeler; B. Hennel; D. Neumeier; P. Luppa


Book ID
103803917
Publisher
Elsevier Science
Year
1991
Weight
308 KB
Volume
572
Category
Article
ISSN
0378-4347

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Urinary porphyrins of porphyric patients were isolated as their methyl esters by using a simple, modified thin-layer chromatographic system. Existing methods for the isocratic ion-pair high-performance liquid chromatographic separation of uroporphyrin and coproporphyrin isomers were decisively impro

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An improved ion-pair reversed-phase high-performance liquid chromatographic system has been developed for the separation of uroporphyrin isomers I, II and III, whereas the isomers III and IV could not be resolved. Application of this method to the analysis of urines from porphyric patients indicated