Application of induced optical activity to the study of protein denaturation
β Scribed by P. Devaux; R. Viennet; M. Legrand
- Book ID
- 115904812
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 500 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0014-5793
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## Abstract To understand the mechanism of ionic detergentβinduced protein denaturation, this study examines the action of sodium dodecyl sulfate on ferrocytochrome __c__ conformation under neutral and strongly alkaline conditions. Equilibrium and stoppedβflow kinetic results consistently suggest t
The particle volume of a protein which represents the expansion of the molecular chain is a useful probe to measure its conformation change in solution. To study the details of the conformation transition, we will report a novel device to trace the particle-volume change against increasing denaturan