Application of high-performance liquid chromatography with a chemiluminescence detection system to determine catecholamines in urine

✍ Scribed by Shin-Ichiro Kobayashi; Jun Sekino; Kazumasa Honda; Kazuhiro Imai

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 443 KB
• Volume: 112
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90266-9

✦ Synopsis

Chemiluminescence generated with the reaction of bis(2,4,6+ichlorophenyl)oxalate and hydrogen peroxide was applied to a detection system for high-performance liquid chromatography to determine fluorescamine-labeled catecholamines. The sensitivity of the chemiluminescence detection system with 25 fmol of detection limit was approximately 20 times higher than that of a conventional fluorescence detection system. Norepinephrine and dopamine in human urine were determined by the use of the new high-performance liquid chromatography detection system with the coefficient of variation of less than 4.0%. Good correlations (r = 0.998 for norepinephrine and r = 0.999 for dopamine) were obtained between the values by the present method and the conventional method.