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Application of gas chromatography-combustion-isotope ratio mass spectrometry for in vivo estimation of 13C metabolic fluxes in microbial culture

✍ Scribed by Y. B. Yuan; T. H. Yang; E. Heinzle


Publisher
John Wiley and Sons
Year
2009
Tongue
German
Weight
44 KB
Volume
81
Category
Article
ISSN
0009-286X

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A method was developed for measuring protein fractional synthetic rates using the N-methoxycarbonylmethyl ester (MCM) derivative of L-[ 1-13C ] valine and on-line gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). The derivatization procedure can be performed rapidly and GC s