## Free flow electrophoresis for the purification of proteins: I. Zone electrophoresis and isotachophoresis The principles and some applications of free flow zone electrophoresis and isotachophoresis are described. The influence of (i) carrier electrolyte conductivity on the migration velocity and
Application of free-flow electrophoresis for isolation and purification of proteins and peptides
β Scribed by Olga I. Loseva; Dr. Alexander V. Gavryushkin; Vladimir V. Osipov; Evgeny N. Vanyakin
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 723 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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## Abstract One of the major expenses associated with recombinant peptide production is the use of chromatography in the isolation and purification stages of a bioprocess. Here we report a chromatographyβfree isolation and purification process for recombinant peptide expressed in __Escherichia coli
A general method for the selective isolation of free and blocked amino-terminal peptides from proteins is described. The rationale behind the methodology is based on the reasoning that if a protein, which has all its free amino groups blocked by citraconylation, is digested with a protease, all pept