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Application of fluorescence polarization to the steady-state enzyme kinetic analysis of calpain II

โœ Scribed by Daniel S. Sem; Patricia A. McNeeley


Book ID
117110258
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
114 KB
Volume
443
Category
Article
ISSN
0014-5793

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General Equation of Steady-State Enzyme
โœ Tetsuya Yomo; Tsuyoshi Yamano; Keizo Yamamoto; Itaru Urabe ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 266 KB

The steady-state velocity equation is derived for the general reaction scheme containing n kinds of enzyme species connected by a network of reversible reaction steps. The general equation is represented by the net rate constants as well as the true rate constants of the individual reaction steps. U