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Application of flow cytometric methods for the routine detection of Cryptosporidium and Giardia in water

✍ Scribed by Graham Vesey; Primrose Hutton; Alan Champion; Nicholas Ashbolt; Keith L. Williams; Andrzej Warton; Duncan Veal

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
649 KB
Volume
16
Category
Article
ISSN
0196-4763

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✦ Synopsis

Cryptosporidium and Giardia are common causes of waterborne disease. The currently used methods of detecting these organisms in water rely on filtration capture, immunofluorescence labelling, and epifluorescence microscopy. These methods are inefficient, labour intensive, and require a highly skilled microscopist. We describe an alternative technique using flocculation concentration, followed by flow cytometry with fluorescence activated cell sorting. Environ-mental samples were analysed, and protozoan-like particles were sorted and collected before confirmation with epifluorescence microscopy. The technique was found to be significantly more sensitive and considerably faster than the conventional methods. o 1994 Wiley-Liss, Inc.

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