Application of factorial design to the optimization of medium composition in batch cultures ofStreptomyces lividansTK21 producing a hybrid antibiotic
✍ Scribed by Sarra Montserrat; Redin Iñaki; Ochin François; Godia Francesc; Casas Carles
- Publisher
- Springer Netherlands
- Year
- 1993
- Tongue
- English
- Weight
- 430 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
The composition of the liquid medium employed to obtain a hybrid antibiotic in batch cultures of a recombinant strain of Sfreptomyces lividans TK21 has been studied. Starch and glutamic acid are the most appropriate carbon and nitrogen sources to support respectively cell growth and antibiotic production. A central composite experimental design has been employed to derive a statistical model of the effect of phosphate and glutamic acid on growth and antibiotic production, and an initial concentration of 10 mM phosphate and 52.8 mM glutamic acid have been found optimal to maximize the final antibiotic concentration in batch cultures.