Application of conducting poly(aniline-co-pyrrole) film to cholesterol biosensor

✍ Scribed by Pratima R. Solanki; Suman Singh; Nirmal Prabhakar; M. K. Pandey; B. D. Malhotra

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 457 KB
• Volume: 105
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.26198

✦ Synopsis

Abstract

Cholesterol oxidase (ChOx) has been covalently immobilized onto poly(aniline‐co‐pyrrole), electrochemically deposited onto indium‐tin‐oxide (ITO) glass plates, using glutaraldehyde as a crosslinker. These poly (An‐co‐Py)/ChOx films have been characterized using UV–visible spectroscopy fourier transform infrared spectroscopy, scanning electron microscopy, and photometric and amperometric techniques, respectively. The poly(An‐co‐Py)/ChOx bioelectrodes have been utilized for cholesterol estimation in the range of 1–10 m__M__. The ChOx activity in poly(An‐co‐Py)/ChOx bioelectrode has been found to be the highest at pH 7.0 at 25°C. The sensitivity and stability of poly(An‐co‐Py)/ChOx bioelectrode have been experimentally determined as 93.35 μA/m__M__ and 10 weeks at 4°C, respectively. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007