โ Scribed by Dr. Wendy Y. Craig; Sue E. Poulin; Preston H. Dorsett; Thomas B. Ledue; Robert F. Ritchie
No coin nor oath required. For personal study only.
In the present study, we have bequn to in-between the results of CBlB and the results vestigate.the possibility of using checkerboard immunoblotting (CBIB) as a semi-quantitative screening tool for detecting human serum IgG against specific viral antigens. The viral antigens studied were Epstein-Barr, herpes simplex I and II, cytomegalovirus, varicella zoster, rubella, rubeola, and mumps. Western immunoblotting experiments using these partially purified preparations demonstrated that there were apparently no interactions between IgG from non-immune sera and the respective viral antigen preparations. The CBlB assay was evaluated using sera of known positive or negative immune status for the viral antigens. There was excellent agreement of alternative methods for evaluating immune status; all discrepancies (1118 sera for mumps, 311 8 sera for rubeola, and 1/28 sera for rubella) involved sera with borderline results, either by CBlB or by the alternative method. Therefore, although further work is required to define the method in terms of sensitivity and clinical specificity, and to refine positive/ negative cutpoint criteria for certain antigen components, our preliminary experience suggests that CBlB has considerable potential in the efficient and inexpensive screening of sera for the presence of IgG against a panel of viral antigens, so as to identify subjects at risk for infection. o 1993 ~i ~e y -~i s s , Inc.
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