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Application of centrifugal partition chromatography coupled with evaporative light scattering detection for the isolation of saikosaponins-a and -c from Bupleurum falcatum roots

✍ Scribed by Kee Dong Yoon; Jinwoong Kim

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
577 KB
Volume
32
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Centrifugal partition chromatography (CPC) coupled with evaporative light scattering detection (ELSD) was applied to separate saikosaponins‐a and ‐c preparatively from Bupleurum falcatum roots. The two‐phase solvent system composed of ethyl acetate/n‐butanol/methanol/water (15:1:3:15 by volume) was used to yield saikosaponins‐a (36.1 mg) and ‐c (28.7 mg) from 370 mg of saponin‐rich extract. The purities of isolated compounds were 96.6 and 97.3% for saikosaponins‐a and ‐c, respectively. Structure identification of these compounds was accomplished by comparison of spectroscopic data of ESI‐MS, ^1^H NMR, and ^13^C NMR with those of previously reported values.

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