Application of Bacterial Artificial Chromosomes to the Generation of Contiguous Physical Maps: A Pilot Study of Human Ryanodine Receptor Gene (RYR1) Region
✍ Scribed by S. Rouquier; M.A. Batzer; D. Giorgi
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 463 KB
- Volume
- 217
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
In order to increase the rate of generation of a contiguous human chromosome 19 physical map, we have investigated the advantages of using a bacterial artificial chromosome (BAC) library in comparison with other systems. This cloning system, recently described, can faithfully propagate DNA fragments greater than 300 kb in size. We have screened a total human genomic BAC library with a complex cosmid probe, specific for the ryanodine receptor gene (RYR1) located at 19q13.1. One (150-\mathrm{kb}) BAC was positive for the ryanodine receptor probe. The ryanodine receptor BAC hybridized to a (150-\mathrm{kb}) overlapping set of nine chromosome 19 -specific cosmids at the 3 ' terminus of the RYR 1 gene as well as two established cosmid contigs which can be linked to the same physical region. The hybridization of the BAC to a (150-\mathrm{kb}) set of chromosome 19 cosmids suggests that this BAC is nonchimeric in structure. rc 1994 Academic Press, Inc.