Application of ascending thin-layer chromatography to Δ5-3-hydroxysteroids of the pregnane series

✍ Scribed by B.P. Lisboa

• Book ID: 104146885
• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 879 KB
• Volume: 39
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97995-6

✦ Synopsis

The chromatographic behaviour of thirty-one d6-3-hydroxy-&,-steroids on analytical Silica Gel G-layers was investigated on eight solvent systems. For the separation of closely related alcoholic trihydroxysteroids, one-dimensional ascending thin-layer chromatography on boric acid-impregnated silica gel layers has been used, Pregnenolone and 16dehydropregnenolone have been resolved by elatography after formation of isonicotinic acid hydrazones. The separation of the epimeric ab-pregnene-3P,zo-diols was only achieved after treatment with 20/3hydroxysteroid-oxidoreductase.