Application of an enzyme-linked immunosorbent assay kit for β-agonist screening of bovine urines in north-eastern Italy
✍ Scribed by R. Angeletti; M. Paleologo Oriundi; R. Piro; R. Bagnati
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 476 KB
- Volume
- 275
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
The apphcatlon and the results of a commercially available enzyme-h&d unmunosorbent assay (ELISA) lat for the screemng of &agomsts are presented The lut was adopted as a screening method because it allows very rapid multi-arm&e detection unthout extraction /3-Agontsts such as clenbuterol, mabuterol, salbutamol and terbutalme can be detected m bovme urme Hrlth a decision bnut of 0 6, 0 9, 16 and 2 5 ng ml-', respectively Confirmation of positwe ELISA results concerning the presence of clenbuterol and salbutamol was made by liquid chromatography and by gas chromatography-mass spectrometry, wth mununoaffinrty clean-up By employmg the described strategy, m a 5-month penod, 59 out of 815 samples were found to be positwe PosItwe groups of samples were from both therapeutic and anabohc use of &agomst drugs Adoptmg 1 ng ml -' clenbuterol aa a cut-off value, the percentage of unconfirmed positwe ELISA herds was 3 6% The rehabdlty of the overall control strategy and of the lut 111 terms of the percentage of false-posltrve results IS evaluated