Application of amino acid analysis by high-performance liquid chromatography with phenyl isothiocyanate derivatization to the rapid determination of free amino acids in biological samples
β Scribed by Vanna Fierabracci; Pellegrino Masiello; Michela Novelli; Ettore Bergamini
- Publisher
- Elsevier Science
- Year
- 1991
- Weight
- 280 KB
- Volume
- 570
- Category
- Article
- ISSN
- 0378-4347
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π SIMILAR VOLUMES
An analytical method for a rapid reverse-phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r > 0.99
## Reversed-phase high-performance liquid chromatography (RP-HPLC) is a powerful method for assaying physiological amino acid concentrations in biological fluids. Four pre-column derivatization methods, with o-phthaldialdehyde (OPA), 9fluorenylmethyl chloroformate (FMOC-Cl), phenyl isothiocyanate