Application of a modified calorimetric enzyme assay to monitor plasma paracetamol levels following single oral doses to non-patient volunteers
✍ Scribed by Peter A.D. Edwardson; John D. Nichols; Keith Sugden
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 405 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0731-7085
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✦ Synopsis
A modified enzyme-based colorimetric method has been used to determine plasma paracetamol profiles following single dose (2 x 500 mg) administration of three dosage forms to non-patient volunteers. The assay is linear over the concentration range 0.15-60 micrograms ml-1 with a coefficient of variation of 9.1% at the 1.5 micrograms ml-1 level. It is rapid, requiring small sample volumes; compares favourably with other techniques such as HPLC; and is not subject to interference from paracetamol metabolites and other drugs. Administration of paracetamol as two different dosage forms, as a solid tablet and as a dispersible tablet, resulted in no statistically significant difference in pharmacokinetic parameters between treatments.