Application of a high-performance liquid chromatographic fluorescence method for the rapid determination of α-tocopherol in the plasma of cattle and pigs and its comparison with direct fluorescence and high-performance liquid chromatography—ultraviolet detection methods.
✍ Scribed by C.H. McMurray; W.J. Blanchflower
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 468 KB
- Volume
- 178
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
High-performance
liquid chromatography is used to develop a sensitive method for the determination of tocopherol levels in the plasma of cattle and pigs. This method is compared with a similar method using UV detection and one using direct fluorescence determination of tocopherol.
Finally a double injection technique used in conjuction with fluorescence detection is shown to enhance the rate of analysis of the tocopherol levels-in bovine plasma extracts_
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UCN-01, 7-hydroxystaurosporine, is an antagonist of protein kinase C, as well as causing cell cycle arrest. We developed and validated an HPLC assay method for the quantitation of UCN-01. Plasma and saliva standard curves were prepared at concentrations ranging from 0.2 to 20.0 mg/mL and 4.0 to 200.