13CΓ13C 2D TOCSY experiments were applied, with both carbon-13 and proton detection, to alleviate the problem of limited 1HΓ1H coherence transfer in certain sugars, e.g. those with the manno or galacto conΓguration. The 13CΓ13C couplings are not as dependent on the di β erent sugar geometries and can
Application of 13C-NMR spectroscopy to In Vitro analysis of enzyme kinetics
β Scribed by H. L. Johnson; D. W. Thomas; M. Ellis; L. Cary; J. I. Degraw
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 281 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0022-3549
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A method is described which allows the identiΓcation of individual carbohydrates of multi-component artiΓcial mixtures using the computer-aided analysis of their 13C NMR spectra, without previous separation. Quantitation of mono-, di-and trisaccharides was carried out after improvement of the experi
## Abstract ^15^N and ^13^C chemical shifts and the ^1^__J__(^15^N^15^N), ^1^__J__(^15^N^13^C) and ^1^__J__(^13^CH) coupling constants have been determined for a number of ^15^Nβenriched cyclic and acyclic secondary nitramines. The results are interpreted in terms of both electronegativity effects
[guanidino-13C]Creatine has been synthesized by reaction involving synthesis of [13C]cyanamide followed by its condensation with sarcosine. The creatine guanidino carbon 13C NMR frequency shifts upon the formation of phosphocreatine by 0.83 ppm with a splitting (2JCP = 3.3 Hz) due to two-bond phosph