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Application de la chromatographie en phase gazeuse à l'étude des nitrilases et amidases

✍ Scribed by J.C. Allageas; A. Arnaud; P. Galzy; A. Arnaud; P. Galzy

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
450 KB
Volume
166
Category
Article
ISSN
1873-3778

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✦ Synopsis

Use of gas-liquid chromatography for the study of nitrilases and amidases

A gas-liquid chromatographic procedure is described for the determination of nitrilasic and amidasic activities. This method allows to monitor the kinetics of the hydrolysis of volatile nitriles and amides and because of its sensitivity, to determine the Michaelis constants K, of the acetonitrilase and the acetamidase of Brevifiacterium R 312. For these enzymes, a correlation is shown between the kinetics monitored by proton nuclear magnetic resonance spectroscopy and gas-liquid chromatography.

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