Apparent effect of pentachloronitrobenzene on the hyphal wall polysaccharides of two strains of Neurospora crassa

✍ Scribed by Prof. Dr. S. G. Georgopoulos

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 698 KB
• Volume: 13
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19730130506

✦ Synopsis

Abstract

A pentachloronitrobenzene (PCNB) resistant mutant of Neurospora crassa and its respective wild type were grown in liquid medium with and without PCNB. Physically isolated hyphal walls were subjected to acid hydrolysis and analyzed for glucose and glucosamine. In the wild type strain PCNB reduced the amount of glucosamine by about 49% and increased the amount of glucose by 15%. The total glucose + glucosamine was significantly higher in the hydrolysates from the PCNB grown walls. These changes were accompanied by striking alterations in the hyphal morphology of the wild type strain but the chemical did not prevent the formation of cell wall around wall‐free protoplasts obtained from this strain. The morphology of the resistant mutant was not affected by PCNB but in the wall hydrolyzates there was a significant reduction of the amound of glucosamine. The findings are discussed in relation to other studies correlating morphology with cell wall composition in N. crassa.