Apparatus for continuous, large-volume suspended cell cultivation

✍ Scribed by R. Fontanges; P. Deschaux; Y. Beaudry

• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 795 KB
• Volume: 13
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260130402

✦ Synopsis

Abstract

A 30‐liter apparatus for growing cells continuously is described. Samples are taken automatically, thus obviating one of the main causes of contamination. In order to eliminate the continuous introduction of a gas stream, the pH was kept constant by means of the injection of NH~4~OH or NaOH. Under these circumstances, and by means of the two methods of agitation described—rotary and “by Vibromixer”—a quantity of cells of approximately 4 × 10^10^ is harvested every 48 to 72 hr.