Apparatus for automatically maintaining cell suspension at low, constant oxygen concentrations: The oxystat

✍ Scribed by Dean P. Jones; Howard S. Mason

• Book ID: 102985201
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 623 KB
• Volume: 90
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90019-2

✦ Synopsis

An apparatus which rapidly provides and maintains constant oxygen partial pressure from 1 to 150 Torr (1.4 to 2 11 ELM) in suspensions of respiring rat hepatocytes (0.50-0.87 pM s-l) was developed. Voltage output from a Clark-type electrode in an incubation vessel regulates oxygen flow into the vessel to control solution oxygen concentration at a predetermined level. After maintenance of cell suspensions (4-15 ml) for 10 to 30 min, metabolism can be quenched and examined.