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APOPTOTIC AND NON-APOPTOTIC MODES OF PROGRAMMED CELL DEATH IN MCF-7 HUMAN BREAST CARCINOMA CELLS

✍ Scribed by Farhana Amin; Ivor D. Bowen; Zsolt Szegedi; Rudolf Mihalik; Bela Szende

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
1011 KB
Volume
24
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

Apoptosis is a specific mode of programmed cell death (PCD), recognized by characteristic morphological and molecular changes. Here we present evidence for a non‐apoptotic type of PCD in human MCF‐7 breast carcinoma cells. We used TNF‐α and tyrphostin AG213 to induce apoptotic and non‐apoptotic cell death respectively in vitro. Microscopic and immunohistochemical studies, together with DNA analysis and flow cytometric analysis of p53 and bcl‐2 oncogene expression, revealed some novel characteristics of non‐apoptotic cell death. We show here for the first time some of the biochemical features of an experimentally induced non‐apoptotic PCD and emphasize the distinct biochemical events leading to apoptotic and non‐apoptotic PCD.

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