Apoptosis of murine BW 5147 thymoma cells induced by dexamethasone and γ-irradiation

✍ Scribed by Inna I. Kruman; Natalia P. Matylevich; Igor P. Beletsky; Vladimir N. Afanasyev; Samuil R. Umansky

• Book ID: 102887028
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 543 KB
• Volume: 148
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041480212

✦ Synopsis

The mode and the kinetics of the death of T-thymoma cells upon dexamethasone treatment and y-irradiation (1 OGy) have been studied using flow cytometry and biochemical analysis. It has been shown that the hormone and y-irradiation induce cell death by apoptosis. In both cases the cells are initially blocked in GJM and die only after overcoming the blockage and cytokinesis. A short exposure to dexamethasone results in a cytostatic effect, whereas a cytotoxic effect is absent. Reducing serum concentration to 2% causes more rapid death both following y-irradiation and dexamethasone. These results are discussed in relation to cell death and proliferation.