Optimization of cell culture conditions
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Shinji Hosoi; Kazunari Murosumi; Katsutoshi Sasaki; Mitsuo Satoh; Hiromasa Miyaj
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Article
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1991
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Springer
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English
โ 637 KB
An expression vector for G-CSF, pASLB3-3, was constructed and introduced into Namalwa KJM-1 cells (Hosoi et al., 1988), and cells resistant to 100 nM of methotrexate (MTX) were obtained. Among them, the highest producer, clone SC57, was selected and the productivity of this clone was further charact