✦ LIBER ✦
Apoptosis in hematopoietic cells is associated with an extensive decrease in cellular phosphotyrosine content that can be inhibited by the tyrosine phosphatase antagonist pervanadate
✍ Scribed by Fridtjof Lund-Johansen; Tom Frey; Jeffrey A. Ledbetter; Peter A. Thompson
- Book ID
- 102651548
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 870 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
In the present study, we investigated whether apoptosis in hematopoietic cells is associated with changes in cellular phosphotyrosine content. Murine thymocytes and B cells, human leukemia cells, and normal peripheral blood leukocytes were induced to undergo apoptosis by treatment with specific stimuli or by incubation in growth factor-deprived medium. hidtiparameter flow cytometry was used to measure changes in phosphotyrosine content that correlated with the appearance of features of programmed cell death, such as cell shrinkage,