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Apoptosis in fetal nucleated erythrocytes circulating in maternal blood

โœ Scribed by Akihiko Sekizawa; Osamu Samura; DongKai Zhen; Vincent Falco; Antonio Farina; Diana W. Bianchi

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
64 KB
Volume
20
Category
Article
ISSN
0197-3851

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โœฆ Synopsis

The purpose of this study was to determine if apoptosis occurs in fetal cells that have crossed into the maternal circulation, which would potentially explain the difference between the number of intact fetal cells and the amount of fetal DNA detectable in maternal plasma. We ยฏow-sorted fetal nucleated erythrocytes (FNRBCs) using antibody to the gamma chain of fetal haemoglobin and conยฎrmed them to be fetal in origin by FISH analysis using chromosome-speciยฎc probes. Fetal cells were then analysed microscopically for the presence of terminal UdTP nuclear end labelling (TUNEL) staining. Apoptotic change was observed in 42.7% of fetal NRBCs (106/246) and 3.5% of maternal cells (29/818). Results of this study indicate that a signiยฎcant number of fetal cells in maternal blood are undergoing apoptosis at the time of sampling. Apoptosis may be one mechanism by which fetal cells are cleared by the maternal circulation.

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