Apolipoprotein E phenotyping by isoelectric focusing in immobilized pH gradients and silver staining
✍ Scribed by Dr. Régine Cartier; Agnès Sassolas
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 631 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0173-0835
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Apolipoproteins C are involved in many ways in the metabolism of plasma lipoproteins. Apolipoproteins C from the delipidated VLDL of 35 controls and 165 normo- and hyperlipoproteinemic patients were analyzed by isoelectric focusing on an immobilized pH gradient, pH 4.0-5.0, with 7 M urea, which rais
A method for isoelectric focusing of apolipoprotein E in an immobilized pH gradient with added carrier ampholytes has been developed. This method is an improvement over conventional isoelectric focusing of apolipoprotein E with respect to resolution, reproducibility, and simplicity. Since monosialo