Apatite precipitation after incubation of biphasic calcium-phosphate ceramic in various solutions: Influence of seed species and proteins
✍ Scribed by Rohanizadeh, R. ;Padrines, M. ;Bouler, J. M. ;Couchourel, D. ;Fortun, Y. ;Daculsi, G.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 817 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
The dissolution-precipitation process for calcium-phosphate ceramics in contact with biological fluid was studied by incubating blocks of biphasic calcium phosphate composed of hydroxyapatite (HA) and -tricalcium phosphate (-TCP) in different solutions: ionic simulated body fluid (SBF) without protein or SBF that contained various proteins and macromolecules separately (fibronectin, vitronectin, albumin, and poly-L-glutamic acid). Transmission electron microscopy studies revealed that apatiteprecipitated microcrystals appeared around ceramic crystals as a result of secondary nucleation; microcrystals were in continuity with the lattice planes of the HA crystals but in a different direction from that of -TCP; the size of the precipitates was smaller when fibronectin, vitronectin, and poly-(L-glutamic acid) were present in SBF as compared to SBF without protein; and fibronectin and vitronectin initiated crystal nucleation in the void spaces between the ceramic crystals.