Aortic endothelial cells synthesize basic fibroblast growth factor which remains cell associated and platelet-derived growth factor-like protein which is secreted
✍ Scribed by Israel Vlodavsky; Rafael Fridman; Robert Sullivan; Joachim Sasse; Michael Klagsbrun
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 766 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Cultured bovine aortic endothelial cells synthesize growth factors which markedly differ in the regulation of their storage and secretion. Endothelial cell lysates, but not conditioned medium, contain a growth factor activity that appears to be basic fibroblast growth factor (FGF) by the following criteria: (1) it elutes from heparin-Sepharose at 1.4-1.6 M NaCI; (2) it is mitogenic for bovine aortic and capillary endothelial cells; (3) it is heat sensitive but stable to dithiothreitol; (4) it has a molecular weight of about 18,000 daltons; and (5) it cross-reacts with antiserum directed against basic FGF. In contrast, endothelial cell conditioned medium, but not lysates, contains a growth factor activity that (1) elutes from heparin-Sepharose at 0.4-0.5 M NaCI; (2) is mitogenic for fibroblasts and vascular smooth muscle cells but not for capillary endothelial cells; (3) is heat stable and dithiothreitol sensitive; and (4) competes with platelet-derived growth factor (PDGF) for binding to fibroblasts. From these criteria, it appears that endothelial cells secrete into the medium growth factors some of which are PDGF-like, but secrete little if any basic FGF. It is suggested that endothelial cell-associated basic FGF acts in an autocrine fashion to stimulate endothelial cell proliferation in response to endothelial cell perturbation or injury. On the other hand, the endothelial cell-secreted growth factors which are smooth muscle cell but not endothelial cell mitogens might exert a paracrine function on neighboring cells of the vessel wall.
Endothelial cells constitute the inner lining of the regulation of their storage and release. Basic fibroblast blood vascular system and play a central role in normal growth factor (FGF), an endothelial cell mitogen, is synhemostasis and in various pathophysiological responses thesized by endothelial cells and remains cell associated. such as acute inflammation, wound healing, tumor an-On the other hand, endothelial cells secrete PDGF and giogenesis, and atherogenesis (Harris-Hooker et al., non-PDGF mitogens which are different than basic FGF.