Antitumor polysaccharides from P. ostreatus (Fr.) quél.: Isolation and structure of a β-glucan
✍ Scribed by Yûko Yoshioka; Ryoko Tabeta; Hazime Saitô; Nobuaki Uehara; Fumiko Fukuoka
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 561 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
We isolated an antitumor glucan (HA /3-glucan) from the neutral polysaccharide fraction (AJ of a hot-water extract of the edible mushroom P. ostreatus (Fr.) Q&l. Purification was accomplished by extractions with 20% sodium chloride solution saturated with thymol and by precipitations with ethanol from dimethyl sulfoxide solution. The glucan showed marked antitumor activity at a dose of 0.1 mglkg. It is a highly branched (1+3)-P-glucan having an average structure represented by a pentasaccharide segment consisting of one nonreducing terminal, one 3,6-di-O-substituted, and three 3-mono-O-substituted P-D-glucopyranosyl residues. This structure was confirmed by examining 13C-n.m.r. spectra taken at 75.46 MHz.