Antisense oligonucleotides to the epidermal growth factor receptor
β Scribed by Lois Witters; Rakesh Kumar; Mahitosh Mandal; C. Frank Bennett; Loren Miraglia; Allan Lipton
- Book ID
- 110232352
- Publisher
- Springer US
- Year
- 1999
- Tongue
- English
- Weight
- 94 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0167-6806
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## Abstract NMuMG cells were incubated with 17Ξ²βestradiol (E)+progesterone (P)+epidermal growth factor (EGF), with or without various types of oligomers (21βmers) to the EGF receptor activity domain (amino acid residues 718 to 724). Sense or antisense oligomers were encapsulated in protein Aβbearin
We have constructed a series of 22 phosphorothioate 20-mer antisense oligonucleotides directed against different regions of the human (EGFR) mRNA. Treatment with EGFR antisense oligonucleotides showed a dose-dependent inhibition of human GEO colon cancer cell growth in soft agar. Western blot analys