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Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

✍ Scribed by Mizuki Kitamatsu; Shunsuke Kurami; Takashi Ohtsuki; Masahiko Sisido

Book ID
104005110
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
353 KB
Volume
21
Category
Article
ISSN
0960-894X

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✦ Synopsis

To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.

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✍ Mizuki Kitamatsu; Masanori Shigeyasu; Mamoru Saitoh; Masahiko Sisido 📂 Article 📅 2006 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 277 KB 👁 1 views

## Abstract A new series of oxy‐peptide nucleic acids (pyrrolidine‐based oxy‐peptide nucleic acids = POPNAs) of four different stereoisomeric forms (__cis__‐L, __cis__‐D, __trans__‐L, __trans__‐D) have been synthesized. To find a favorable stereoisomer of POPNA for hybridization with DNA or RNA, th