Antimutagenic activity of chemical fractions isolated from a commercial soybean processing by-product

✍ Scribed by Michael J. Plewa; Elizabeth D. Wagner; Mark A. Berhow; Adam Conway; A. Lane Rayburn; Diana Anderson

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 122 KB
• Volume: 19
• Category: Article
• ISSN: 0270-3211
• DOI: 10.1002/(sici)1520-6866(1999)19:2<121::aid-tcm5>3.0.co;2-o

✦ Synopsis

Commercial products of agronomic crop plants may become a reliable and inexpensive source of phytonutrients, such as antimutagenic food supplements. We previously demonstrated that PCC, an ethanol extract of a commercial soybean processing by-product, was able to repress induced genomic DNA damage, whole cell clastogenicity, and point mutation in mammalian cells. In this paper we separated PCC into a series of chemically defined fractions and determined their ability to repress induced mutagenic damage in Chinese hamster lung cells, Chinese hamster ovary cells and human lymphocytes. Fraction PCC70 (PCC 70% methanol eluate) contained the flavonoids from PCC and daidzin and genistin repressed 2-acetoxyacetylaminofluorene (2AAAF)-induced DNA damage measured with single cell gel electrophoresis. Genistein, however, enhanced the genotoxic impact of 2AAAF. Fraction PCC100 (PCC 100% methanol eluate) had the greatest level of antigenotoxic activity against 2AAAF in CHO cells and repressed the genotoxic capacity of the dietary carcinogen 2-amino-3-methylimidazo-(4,5-f)quinoline (IQ) in human lymphocytes. These data indicate that commercial soybean products and by-products may be a source of chemoprotective food additives.