Antigenotoxic potential of Gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line

✍ Scribed by K.M. Ramkumar; L. Sankar; C. Manjula; K. Krishnamurthi; S. Saravana Devi; T. Chakrabarti; K. Kalaiselvi; M. Palanivel; P. Rajaguru

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 391 KB
• Volume: 51
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/em.20543

✦ Synopsis

In this study we have evaluated the genoprotective effect of the ethanol extract of Gymnema montanum (GLEt) leaves in human peripheral blood lymphocytes and HL-60 cell line in vitro using the comet assay. DNA damage was induced by treating the cells with H 2 O 2 and methyl methane sulphonate (MMS). GLEt treatment effectively protected the lymphocytes and HL-60 cell line from H 2 O 2 -induced oxidative DNA damage in a dosedependent manner whereas it was not effective against alkylative DNA damage caused by MMS. The global percent repair efficiency also showed that both pre-and post-GLEt treatment provided effective protection against H 2 O 2 induced DNA damage but not as effective against MMS. At 200 lg ml 21 level, its repair capacity against H 2 O 2 induced DNA damage was comparable to that of vitamin-C (100 lM). Furthermore, exposure to GLEt reduced the formation of apoptotic cells caused by H 2 O 2 , which was demonstrated by the decreased sub-G1-DNA content in cell cycle analysis and apoptotic frequencies of lymphocytes in an annexin-V binding assay. In addition, GLEt was found to have effective peroxide scavenging ability in dose-dependent manner. The protective efficiency of the extract was found to be directly proportional to its total phenolic content. The present study indicates that G. montanum leaves are a significant source of phytochemicals with antigenotoxic and antioxidant activity, and thus has potential therapeutic use.