Rabbit antisera to homogenates of unfertilized sea urchin eggs were absorbed with pluteus extract. In the same way, antisera to gastrulae or plutei were also made. "stage specific" by absorption with extracts of eggs. When tested by means of immunoelectrophoresis the absorbed sera formed precipitate
Antigenic specificity of the sea urchin sperm surface
✍ Scribed by M. Krajnović; S. Kečkeš
- Publisher
- Springer-Verlag
- Year
- 1967
- Tongue
- English
- Weight
- 224 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0025-3162
No coin nor oath required. For personal study only.
✦ Synopsis
The antigenic composition of the sperm surface in Para. centrotus lividus Lain. and Sphaereehinus granularis Lain. was studied using the sperm agglutination test with homologous and heterologous antisera. The results indicated that sperm of both species have at least one antigenic determinant located on their surface and shared by both species. In addition, Paracentrotus sperm bear at least one species specific determinant on their surface. The existence of antigenic determinants common to gametes of both species was demonstrated.
📜 SIMILAR VOLUMES
## Abstract In this study we used Triton X‐100 extracted sea urchin spermatozoa to investigate the mechanical behavior of the basic 9+2 axoneme. The dynein motors were disabled by vanadate so that the flagellum is rendered a passive structure. We find that when a proximal portion of the flagellum i
## Abstract A sperm‐activating substance (SAS) was obtained from the jelly coat of sea‐urchin ova and its chemical properties were investigated in three seaurchin species. The SAS was partially purified from the jelly coat of __pseudocentrotus__ eggs through several steps of purification by procedu
A high-resolution sodium dodecyl sulfate polyacrylamide gel electrophoresis system has been used to show the presence, in both whole sperm and isolated flagellar axonemes, of eight polypeptides migrating in the 300,000--350,000 molecular weight range characteristic of the heavy chains of dynein ATPa