✦ LIBER ✦

Antigenic modulation and internalization of monoclonal antibody to human colonic carcinoma cells detected by enzyme-linked immunosorbent assay

✍ Scribed by T. Yokota; E. Otsuji; A. Noguchi; T. Yamaguchi; K. Sawai; T. Takahashi

Publisher: John Wiley and Sons
Year: 1989
Tongue: French
Weight: 460 KB
Volume: 44
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910440626

No coin nor oath required. For personal study only.

✦ Synopsis

We studied antigenic modulation and internalization of monoclonal antibody (MAb) A7 using the enzyme-linked immunosorbent assay (ELISA) and biotin-labelled antibodystaining techniques. Incubation of the colonic SWI I 16 cell line with an excess of MAb A7 induced modulation of the cellsurface antigen. When the line was assayed by ELISA, a change in cellular reactivity with MAb A7 was seen after I hr. After 24 hr, the cellular reactivity showed a 52% decrease in absorbance. Modulation was inhibited by 0. I% sodium azide and acetone fixation, suggesting that this is an energydependent phenomenon. The internalization of biotinylated MAb A7 was examined. Internalized biotinylated MAb A7 was detected in cells fixed before labelling with avidin-biotin peroxidase complex. It was observed that the amount of MAb A7 which remained associated with the cell surface had decreased since A7 was internalized.

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