Antigen–antibody recognition by Fourier transform IR spectroscopy/attenuated total reflection studies: Biotin–avidin complex as an example
✍ Scribed by R. Barbucci; A. Magnani; C. Roncolini; S. Silvestri
- Book ID
- 101720296
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1991
- Tongue
- English
- Weight
- 477 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Biotin-avidin recognition is studied by Fourier transform ir spectroscopy /attenuated total reflection (FTIR/ ATR) under physiological conditions. The ureido portion of biotin is confirmed to be involved in the interaction with avidin, as previously found, but when the biotin-avidin complex forms, an electrostatic interaction occurs between the carboxylate group of the biotin molecule and the protonated aminic end group of the avidin amino acid side chains. Comparison of the biotin-avidin system with the biotin-1,4-diaminobutane and biotin-tryptophan systems confirms these findings.