Antifungal activity of resveratrol oligomers from Cyphostemma crotalarioides

✍ Scribed by Bala, Adil E; Kollmann, Albert; Ducrot, Paul-Henri; Majira, Amel; Kerhoas, Lucien; Delorme, Robert; Einhorn, Jacques

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 151 KB
• Volume: 55
• Category: Article
• ISSN: 1526-498X
• DOI: 10.1002/(sici)1096-9063(199902)55:2<206::aid-ps871>3.0.co;2-z

✦ Synopsis

Figure 2. Structures of neuroactive s ubs tances from Liquidamber s tyraciflua and L . formos ana .

Indonesian plants, as well as many plants collected in J apan were screened for the presence of neuroactive substances ; they showed a variety of proüles of the nerve responses. Among them, Liquidamber styraciýua L and L formosana Hance were chosen for investigating the isolation of active principles in this study. This summary reports the occurrence in the immature fruits of these plants of active components which increased impulse frequency remarkably, and also the isolation and identiücation of such active components. The active compound 1 (20 mg) was isolated from the hexane-soluble fraction of the methanol extract of L styraciýua (4.4 kg) by using conventional separation methods, such as silica gel column chromatography, preparative TLC etc, to give the pure compound. Spectral analyses, as well as a literature survey and chemical synthesis from commercially available betulin, allowed identiücation of the active compound 1 as betulonic acid, a triterpene acid.3

The active compound 2 (2 mg) was obtained from the hexane-soluble fraction of the methanol extract of L formosana (78 kg) by similar procedures, and the active compound 3 (14 mg) from the ethyl-acetate soluble fraction of the same extract. By comparison of their UV, IR, [1H] and [13C] NMR and MS spectral data with those reported in the literature,4h6 the active compounds 2 and 3 were identiüed as 1methoxy-9-caryolanol and eudesm-4(14)-ene-1,6diol, respectively, which are both sesquiterpene alcohols.