Anticircular high performance thin-layer chromatography

✍ Scribed by Kaiser, R. E.

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 690 KB
• Volume: 1
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240010309

✦ Synopsis

Abstract

The principal analytical details for the third of three possible modes in high performance thin‐layer chromatography are given, namely the anticircular mode. Separation is achieved by allowing the mobile phase to enter the plate layer on a precise outer circle line, from where it flows towards the centre with nearly constant speed. This technique is theoretically and practically the fastest of all three possible in HPTLC. It permits maximum sample capacity with a minimum of time, layer and mobile phase consumption. It is therefore the most economical HPTLC technique.

A new carrier‐free mobile phase transfer principle is used. The conditions for qualitative and quantitative analysis are good: repeatability, reproducibility and accuracy of routine TLC analyses are superior to those achieved by the classical trough technique. The specially narrow spot‐path in anticircular HPTLC facilitates automated quantitation. Compared with the linear and circular modes, the anticircular mode shows better separation and significantly increased sensitivity at higher Rf‐values. The drawback, however, is that the separation power (expressed by the separation number) is lower compared with the other two modes.