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Anticancer effect of photodynamic therapy with hexenyl ester of 5-aminolevulinic acid in oral squamous cell carcinoma

✍ Scribed by Yeon-Hee Moon; Jong-Hwan Park; Soo-A Kim; Jee-Bum Lee; Sang-Gun Ahn; Jung-Hoon Yoon

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
261 KB
Volume
32
Category
Article
ISSN
1043-3074

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✦ Synopsis

Abstract

Background.

Five‐aminolaevulinic acid (ALA) and its derivatives act as precursors of the photosensitizer protoporphyrin IX (PpIX). In this study, the effect of photodynamic therapy (PDT) with hexenyl ester of ALA (ALA‐hx) was examined in a human oral squamous cell carcinoma, YD10B cells.

Methods.

PpIX accumulation and mRNA expression of coproporphyrinogen oxidase (CPO) by ALA and ALA‐hx was examined. Cell viability was examined by MTT assay and the molecular mechanism was investigated.

Results.

The PpIX synthesis and mRNA expression of CPO was much higher in the cells treated with ALA‐hx than ALA. At the concentration that PDT with ALA did not affect cell growth, ALA‐hx PDT effectively produced reactive oxygen species (ROS) and suppressed cell growth. Growth inhibition by ALA‐hx PDT was due to mitochondrial‐dependent apoptosis.

Conclusion.

Our results suggest that ALA‐hx PDT effectively induced apoptosis of YD‐10B cells and can be considered as a therapeutic alternative for oral cancer. © 2009 Wiley Periodicals, Inc. Head Neck, 2010

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