A novel and rapid cloning method for the
✍
Yasushi Uematsu
📂
Article
📅
1991
🏛
Springer-Verlag
🌐
English
⚖ 536 KB
Conventional polymerase chain reactions (PCR) require sequence information on both ends of the DNA to be amplified. The novel technique described here allows the amplification of cDNA fragments with sequence information from one end only. Blunt-ended double-strand cDNA is prepared, circularized with