Antibody Engineering: Methods and Protocols

✍ Scribed by Damien Nevoltris; Patrick Chames

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 536
• Edition: Hardcover
• Category: Library

✦ Synopsis

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

✦ Table of Contents

Front Matter ....Pages i-xvi
Front Matter ....Pages 1-1
Antibody Design and Humanization via In Silico Modeling (Vinodh B. Kurella, Reddy Gali)....Pages 3-14
Antibody Affinity Maturation by Computational Design (Daisuke Kuroda, Kouhei Tsumoto)....Pages 15-34
Use of IMGT® Databases and Tools for Antibody Engineering and Humanization (Marie-Paule Lefranc, François Ehrenmann, Sofia Kossida, Véronique Giudicelli, Patrice Duroux)....Pages 35-69
Front Matter ....Pages 71-71
Construction of Human Naïve Antibody Gene Libraries (Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, Katia Scotlandi)....Pages 73-91
Construction of Synthetic Antibody Libraries (Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau)....Pages 93-108
Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments (Doreen Könning, Steffen Hinz, Julius Grzeschik, Christian Schröter, Simon Krah, Stefan Zielonka et al.)....Pages 109-127
Display Technologies for Generation of Ig Single Variable Domains (Vladimir Bobkov, Bas van der Woning, Hans de Haard)....Pages 129-144
A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries (Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir et al.)....Pages 145-161
Front Matter ....Pages 163-163
Phage Display and Selections on Purified Antigens (Magali Colazet, Patrick Chames)....Pages 165-178
Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display (Martina L. Jones, Stephen M. Mahler, Sumukh Kumble)....Pages 179-195
Selection of Antibody Fragments Against Structured DNA by Phage Display (Mahdi Zeraati, Marcel E. Dinger, Daniel Christ)....Pages 197-209
Selection of Antibody Fragments by Yeast Display (Nathalie Scholler)....Pages 211-233
Rapid Selection of High-Affinity Antibody scFv Fragments Using Ribosome Display (Birgit Dreier, Andreas Plückthun)....Pages 235-268
In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display (Naoto Nemoto, Shigefumi Kumachi, Hidenao Arai)....Pages 269-285
Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood (Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ et al.)....Pages 287-309
Front Matter ....Pages 311-311
Expression of IgG Monoclonals with Engineered Immune Effector Functions (Rodrigo Vazquez-Lombardi, Damien Nevoltris, Romain Rouet, Daniel Christ)....Pages 313-334
An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High-Level Monoclonal Antibody Producing CHO DG44 Cell Lines (Jessna H. M. Yeo, Mariati, Yuansheng Yang)....Pages 335-349
Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins (Martin Siegemund, Nadine Beha, Dafne Müller)....Pages 351-364
Front Matter ....Pages 365-365
Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases (Chao Li, Tiezheng Li, Lai-Xi Wang)....Pages 367-380
Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity (Christian Kellner, Stefanie Derer, Katja Klausz, Sophia Rosskopf, Tim Wirt, Thies Rösner et al.)....Pages 381-397
Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High-Affinity Interaction with FcRn at pH 6.0 (Abhishek Saxena, Bingxin Bai, Shin-Chen Hou, Lianlian Jiang, Tianlei Ying, Shane Miersch et al.)....Pages 399-417
Front Matter ....Pages 419-419
Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance (Stephen Hearty, Paul Leonard, Hui Ma, Richard O’Kennedy)....Pages 421-455
Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development (Edward Franklin, Orla Cunningham, Brian Fennell)....Pages 457-477
The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies (Peter M. Bowers, William J. Boyle, Robert Damoiseaux)....Pages 479-489
Selection and Use of Intracellular Antibodies (Sandrine Moutel, Clément Nizak, Franck Perez)....Pages 491-503
Site-Specific Radioactive Labeling of Nanobodies (Maxine Crauwels, Sam Massa, Charlotte Martin, Cecilia Betti, Steven Ballet, Nick Devoogdt et al.)....Pages 505-540
Back Matter ....Pages 541-543