𝔖 Bobbio Scriptorium
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Antibody Covalent Immobilization on Carbon Nanotubes and Assessment of Antigen Binding

✍ Scribed by Enrica Venturelli; Chiara Fabbro; Olivier Chaloin; Cécilia Ménard-Moyon; Cristian R. Smulski; Tatiana Da Ros; Kostas Kostarelos; Maurizio Prato; Alberto Bianco

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
780 KB
Volume
7
Category
Article
ISSN
1613-6810

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✦ Synopsis

Abstract

Controlling the covalent bonding of antibodies onto functionalized carbon nanotubes is a key step in the design and preparation of nanotube‐based conjugates for targeting cancer cells. For this purpose, an anti‐MUC1 antibody (Ab) is linked to both multi‐walled (MWCNTs) and double‐walled carbon nanotubes (DWCNTs) using different synthetic strategies. The presence of the Ab attached to the nanotubes is confirmed by gel electrophoresis and thermogravimetric analysis. Most importantly, molecular recognition of the antigen by surface plasmon resonance is able to determine similar Ab binding capacities for both Ab–DWCNTs and Ab–MWCNTs. These results are very relevant for the design of future receptor‐targeting strategies using chemically functionalized carbon nanotubes.

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