Antibody Arrays: Methods and Protocols

✍ Scribed by Kelly C. Whittaker, Ruo-Pan Huang

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 319
• Series: Methods in Molecular Biology 2237
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody arrays pertaining to investigations of specific research topics and biological processes as well as guidance on the methods of processing, analysis, and storage of array data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Antibody Arrays: Methods and Protocols aims to empower the reader with the information required to select the most appropriate array for their research application, with the technical knowledge to use and process the array, and with the knowledge to perform analysis that realizes the maximum benefit from the data generated.

✦ Table of Contents

Front Matter ....Pages i-xi
Sandwich-Based Antibody Arrays for Protein Detection (Ying Qing Mao, Brianne Petritis, Ruo-Pan Huang)....Pages 1-10
Well-Based Antibody Arrays (Robert S. Matson)....Pages 11-38
One-Step Antibody Arrays (Ying Qing Mao, Lin-Hai Li)....Pages 39-44
Global Detection of Proteins by Label-Based Antibody Array (Zhizhou Kuang, Li-Pai Chen, Ruochun Huang, Ruo-Pan Huang)....Pages 45-53
Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen (Anastasiia Halushkina, William Buchmann, Nathalie Jarroux, Régis Daniel)....Pages 55-67
Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays (Abby Jones, Patricia Czarnecki, Lasangi Dhanapala, James F. Rusling)....Pages 69-82
Cytometry Multiplex Bead Antibody Array (Benyue Zhang, Gang Xiao, Ying Qing Mao, Zhiqiang Lv, Ruo-Pan Huang)....Pages 83-92
Antibody Arrays: Barcode Technology (Liwei Yang, Jun Wang)....Pages 93-102
Reverse Phase Protein Arrays (Justin B. Davis, Sydney Andes, Virginia Espina)....Pages 103-122
Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression (Ruochun Huang, Chun-Hui Gao, Wenjuan Wu, Ruo-Pan Huang)....Pages 123-128
Methods for Membranes and Other Absorbent Surfaces (Jarad J. Wilson, Yun Xi, Ruochun Huang, Ruo-Pan Huang)....Pages 129-139
Precise Chip-to-Chip Reagent Transfer for Cross-Reactivity-Free Multiplex Sandwich Immunoassays (François Paquet-Mercier, David Juncker, Sébastien Bergeron)....Pages 141-149
Antibody Printing Technologies (Valentin Romanov, Benjamin D. Brooks)....Pages 151-177
Self-Assembling Peptide Hydrogels for 3D Microarrays (Greta Bergamaschi, Alessandro Strada, Roberto Frigerio, Marina Cretich, Alessandro Gori)....Pages 179-189
Surface Modification of Glass Slides with Aminosilanes for Microarray Use (Chris Stuart)....Pages 191-198
Dried Blood-Based Protein Profiling Using Antibody Arrays (Kelly C. Whittaker, Ying Qing Mao, Siwei Zhu, Zhiqiang Lv, Ruo-Pan Huang)....Pages 199-206
Profiling Glycoproteins on Functionalized Reverse Phase Protein Array (Ying Zhang, Liyuan Zhang, Anton Iliuk, W. Andy Tao)....Pages 207-215
Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin (Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike)....Pages 217-224
Analyzing Signaling Pathways Using Antibody Arrays (Hao Tang, Chaohui Duan, Zhizhou Kuang, Ruo-Pan Huang)....Pages 225-236
Using Antibody Arrays for Biomarker Discovery (Shuhong Luo, Yunbiao Ling, Li-Pai Chen, Ruo-Pan Huang)....Pages 237-245
Immune Cell Isolation from Murine Intestine for Antibody Array Analysis (Joshua A. Owens, Rheinallt M. Jones)....Pages 247-256
Database Development for Antibody Arrays (Zhaowei Xu, Likun Huang, Sheng-Ce Tao)....Pages 257-261
Data Analysis for Antibody Arrays (Huihua Zhang, Ying Qing Mao, Brianne Petritis, Ruo-Pan Huang)....Pages 263-276
Statistical Analysis Options for Antibody Array Data (Jingqiao Lu)....Pages 277-314
Back Matter ....Pages 315-316

✦ Subjects

Life Sciences; Biological Techniques; Chemistry/Food Science, general