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Antibodies to different peptides in osteopontin reveal complexities in the various secreted forms

✍ Scribed by Shigeyuki Kon; Masahiro Maeda; Tatsuya Segawa; Yoshiaki Hagiwara; Yuko Horikoshi; Shunsuke Chikuma; Kumiko Tanaka; Mohammod Mizanur Rashid; Manabu Inobe; Ann F. Chambers; Toshimitsu Uede

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
332 KB
Volume
77
Category
Article
ISSN
0730-2312

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✦ Synopsis

We have generated synthetic peptides corresponding to various portions of human osteopontin (OPN) and have immunized rabbits and mice with these peptides to generate polyclonal and monoclonal antibodies specific to human OPN. We then generated six distinct sandwich enzyme-linked immunoabsorbent assay (ELISA) systems by using different pairs of polyclonal and monoclonal antibodies against human OPN. These systems allowed us to detect not only various isoforms and truncated forms of recombinant OPN, but also the glycosylated form of native urinary OPN. Most importantly, tumor-derived OPN was differentially detected by the six ELISA systems. The ELISA systems that we have developed will be useful for clarifying the functional roles for OPN in vivo in various physiologic and pathologic conditions.

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